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KMID : 0880220130510060829
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Journal of Microbiology
2013 Volume.51 No. 6 p.829 ~ p.835
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Reduction of Organic and Inorganic Selenium Compounds by the Edible Medicinal Basidiomycete Lentinula edodes and the Accumulation of Elemental Selenium Nanoparticles in Its Mycelium
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Elena Vetchinkina
Ekaterina Loshchinina
Viktor Kursky
Valentina Nikitina
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Abstract
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We report for the first time that the medicinal basidiomycete Lentinula edodes can reduce selenium from inorganic sodium selenite (SeIV) and the organoselenium compound 1,5- diphenyl-3-selenopentanedione-1,5 (DAPS-25) to the elemental state, forming spherical nanoparticles. Submerged cultivation of the fungus with sodium selenite or with DAPS- 25 produced an intense red coloration of L. edodes mycelial hyphae, indicating accumulation of elemental selenium (Se0) in a red modification. Several methods, including transmission electron microscopy (TEM), electron energy loss spectroscopy (EELS), and X-ray fluorescence, were used to show that red Se0 accumulated intracellularly in the fungal hyphae as electron-dense nanoparticles with a diameter of 180.51±16.82 nm. Under designated cultivation conditions, shiitake did not reduce selenium from sodium selenate (SeVI).
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KEYWORD
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shiitake, elemental selenium, nanospheres, intracellular synthesis
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